1.
Pathology And Serum Biochemistry Of Layers Affected With Fatty Liver Hemorrhagic Syndrome
by Ahmad Jawad Sabir | Dr. Asim Aslam | Dr. Muhammad | Dr. Muhammad Yasin Tipu.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Fatty liver syndrome is a non-infectious disease of chickens, primarily cage layers, characterized by enlarged, fat-infiltrated livers, with or without subcapsular hemorrhages and excessive abdominal fat deposits. Excessive caloric intake is thought to be one causative factor, and the disease occurs frequently after molting when caloric intake is high. Cage layers move less, using less energy which may contribute to the problem. The present project was designedto study the serum biochemical and pathological changes in the laying hens affected with fatty liver hemorrhagic syndrome and possible dietary sources responsible for this syndrome.
For this purpose healthy and diseased birds from commercial poultry farms having problem of FLHS in their flocks were selected. Three types of samples i.e. blood, tissue and feed samples were collected. Serum samples were processed to quantify concentration of different plasma enzymes including ALT, AST, triglycerides, serum cholesterol, glucose and LDH levels by using standard protocols. Values obtained from both healthy and diseased birds were compared. While liver samples were collected from both diseased and healthy birdsfor study of gross and microscopic changes in their structure. Proximate analysis of feed samples which were collected from each farm having this problem was conducted to check the level of fats and carbohydrates. Data was statistically analyzed by applying student t-test.
The average concentration of serum glutamic pyruvic transaminase (SGPT),serum glutamic oxaloacetate transaminase, triglycerides, serum cholesterol, glucose and lactate dehydrogenase were 10.78±2.01 (IU/L), 195.5±28.93 (IU/L), 69.2±5.14 (mg/dl), 217.52±23.63 (mg/dl), 205.14±19.6 (mg/dl) and 2466.06±336.2 (IU/L) respectively in diseased birds. The corresponding values in the healthy birds were 4.12±1.7 (IU/L), 81.16±6.83 (IU/L), 59.44±4.92 (mg/dl), 131.94±8.93 (mg/dl), 188.38±13 (mg/dl) and 1706.64±244.37 (IU/L) respectively. The activities of SGPT, SGOT, serum cholesterol and LDH were significantly higher in diseased than in healthy birds (p<0.05). Some birds in diseased group showed 2-3 times greater concentration of plasma enzymes as compared to healthy birds. For the definitive diagnosis of this problem histopathological studies were conducted on liver samples. Liver from healthy birds showed no changes while diseased birds showed both gross and microscopic changes. Feed samples were collected from respective farms and proximate analysis was conducted. Average fat and carbohydrate content recorded for feed samples was 6.44±1.43% and 57.75±3.79% respectively. While feed analysis of control poultry diets showed a significant difference having fat 03.21±0.89% and NFE was55.77±1.67%.
It was concluded from this study that selected parameters of serum biochemistry including SGPT, SGOT, serum cholesterol, triglycerides and lactate dehydrogenase could be useful in the diagnosis of FLHS. There is significant increase in concentration of these enzymes in blood of affected birds specially laying hens. Macroscopic and microscopic changes can also be observed but it will lead to delayed diagnosis of this syndrome. Furthermore balanced feed will be helpful in prevention of this problem while farmers and poultry practitioners can seek help through proximate analysis of feed samples that can help in prevention of this metabolic problem.
Availability: Items available for loan: UVAS Library [Call number: 1609,T] (1).
2.
Study On Effect Of Nigella Sativa (Kalonji)Alone And In Combination With Allium Sativum (Garlic) On Histopathology of Tissues Induced by Newly Emerged Newcastle Disease Virus in Broilers
by Saira jabeen | DR. Muhammad Yasin tipu | Dr.Hassan Mushtaq | Ishtiaq ahmad.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1921,T] (1).
3.
Comparative Hemato-Biochemical Study On Theileriosis In Naturally Infested Punjab Urial (Ovis Orientalis Punjabiensis) And Domestic Sheep (Ovis Aries)
by Sammuel Shahzad | Dr. Muhammad Yasin Tipu | Dr. M. Ijaz | Mr. Irfan Irshad.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1981,T] (1).
4.
A Study On Immunomodulatory Effect Of Nigella Sativa And Allium Sativum Against Escherichia Coli Infection In Broiler Birds
by Fiza Ahmad | Dr Muhammad yasin tipu | Dr. Muti-ur- rehman khan | Prof. Dr.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2022,T] (1).
5.
Esponse Of Lymphatic Tissues To Dietary Supplementation Of Nigella Sativa And Curcuma Longa In Broilers Challenged With Pasteurella Multocida (strain A)
by Muhammad Akmal raheem | DR. Muhammad Yasin tipu | Dr. Muti ur rehman khan | Prof. Dr.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2050,T] (1).
6.
Effect Of Nigella Sativa On Embryonated Eggs Experimentally Infected With Newcastle Disease Virus
by Arif Ullah Khan (2013-VA-561) | Dr. Muhammad Yasin Tipu | Dr. Raheela Akhtar | Dr. Ali Ahmad Shiekh.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: In Agriculture Industry of Pakistan poultry sector plays a vigorous, animated and a well
known role. There are more than 1.5 million peoples whose financial gain and employments are
related directly or indirectly to this sector which provides livelihood to about 60 % rural
inhabitants. Meat and eggs are the products of poultry industry. In Gross Domestic Products its
constant cost factor is 1.2%, in livestock is 10.4% and in agriculture it contributes 5.76%.In the
total meat production quantity of the country, poultry meat contribution is 26.8 % (Anonymous
2013).
The member of family Paramyxoviridae, Newcastle disease virus (NDV) contains six genes,
single stranded RNA genome without segment and have different strains arranged as virulent
which is velogenic, intermediate virulent mesogenic and less virulent lentogenic. The Newcastle
disease or pseudo-fowl pest is complete destructive having high death ratio in poultry. The NDV
attacks on respiratory system causes distress, produce nervous disorders, disturb gastrointestinal
tract and causes hemorrhagic lesions in intestine, downward change in level of egg production
and high mortality (Alexander 2000).
In embryonated eggs the cells attached with allantoic cavity help to grow and develop the
NDV virus which is inoculated for the purpose of propagation. In approximately 24 hours post
inoculation the virus reach higher concentration. First of all the virus destroys the live cells in
which it is growing and then it is widely spread by the allantoic fluid. If the inoculated strains of
virus are virulent i:e velogenic, embryos die within two days post inoculation mostly. The
embryos which are dead or alive show histopathological and gross changes remarkably caused
by the inoculated NDV (Al-Garib et al. 2003).
Introduction
2
To evaluate the value or determine the pathogenicity of the virus or to propagate Newcastle
disease virus the embryos of chicken have been usually used. The NDV strains which are
virulent means velogenic, kill the embryo within 48 hours, and lentogenic or less
virulent(mesogenic) take 5 to 7 days to kill either the embryos or may not kill at all (Lam et al.
1995).
It always had an inherited pattern of thoughts to advance the progress in the process of
healing by using herbal plants in all the civilizations. All over the world, to provide treatment for
diseases in daily life medicinal herbal plants and seeds have been used since hundreds of year
ago and in ahead of times. Plants play important role and serves as a base in the development of
drugs (Ates and Turgay 2003).
For the promotion and well being of health of both animals and humans, since thousands of
year ago black cumin seeds which is commonly known as black seed are used. It is stated by
Prophet Muhammad (P.B.U.H) that black seeds are treatment for every disease except the final
state the death. Its scientific name is Nigella sativa and belongs to the family Ranunculaceae.
The black seeds contain the main active and wider in scope ingredient named as crystalline
nigellone. The composition of other ingredients which are present in these seeds are proteins,
vitamins B1, B2, B3, oleic acid, myristic acid, linolic acid, folic acid, thymoquinone, palmitic
acid, beta sitosterol and stearic acid. In black seed the main elements are phosphorous, iron, zinc,
calcium, and copper. Anti-bacterial, anti-parasitic, anti-tumor, anti-hypertensive, immunestimulator,
anti-inflammatory and anti-histamine are the main effects of black cumin seeds
(AitMbarek et al. 2007).
Black cumin seeds are a hypothetical remedy for all ills, diseases and disorders, but not to
stop growing old and death from happening. In Bible it is identified as black cumin and tending
Introduction
3
to restore health (Worthen et al. 1997).
The antiviral drugs are not usually used in poultry industry due to economic problems
and may cause toxicity, but some drugs shows effect against virus which effect poultry. The
antiviral drug Ribavirin was used to compare its effect with a herbal plant to see which one is
effective. Higher doses produce toxicity and also were the cause of death. The 20μl/ml dose
showed the significant effect and the embryoes remain live after experimentally infected with
Newcastle disease virus (Omer et al. 2014). Availability: Items available for loan: UVAS Library [Call number: 2255-T] (1).
7.
Effect of Curcuma Longa on Embryonated Eggs Experimentally Infected With Avian Influenza Virus
by Syed Iftikhar Ali Shah (2013-VA-436) | Dr. Muhammad Yasin Tipu | Prof. Dr. Asim Aslam | Prof.Dr.Muhammad Sarwar Khan.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: CD Error Availability: Items available for loan: UVAS Library [Call number: 2264-T] (1).
8.
Study On Pathogenesis Of Mixed Infection Of Infectious Bronchitis Virus (Ibv) And Avian Influenza Virus (H9n2) In Experimentally Infected Broiler Chicken
by Arsala Khan (2013-VA-605) | Prof. Dr. Asim Aslam | Dr. Muhammad Yasin Tipu | Prof. Dr. Khushi Muhammad.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2015Dissertation note: Avian influenza and infectious bronchitis are the most devastating contagious and viral diseases, affected poultry in Pakistan and many other Asian countries. Regarding the virulence, Avian influenza may be low, or high pathogenic. Mainly due to its pandemic potential and contagious nature, the low pathogenic avian influenza virus (H9N2) is of major concern in poultry industry especially in Pakistan. Co-infection of poultry with more than one bacterial and /or viral agent is common in field especially in our country due to inadequate biosecurity measures which causes high mortality and confusing the diagnosis of these viruses. Hence the present project was designed to study the pathogenesis of mix infection of IBV and H9N2 through gross, histopathological lesions and antibody titer in experimentally infected broiler chicken. For this purpose a total of 80 specific pathogen free chicks were procured, randomly divided into four groups A, B, C and D; and raised under uniform managmental condition in experimental sheds of UVAS Lahore. Group A and B were intarnasally challenged with 0.2 ml infectious bronchitis virus (EID50 = 104.5 per 0.1 ml) at 23rd day of age, likewise group B and C were inoculated with 0.2 ml of H9N2 (EID50 = 106 per ml) at 26th day of age while, group D served as a negative control group. After infection the clinical signs, feed consumed and body weight gain were regularly monitored, the serum samples taken on days 23rd, 26th, 29th, 35th, and 40th day of age were tested for HI antibody titer simultaneously, the necropsy of birds (n=3) sacrificed were conducted to observe the gross lesions, tissue samples including lungs, liver, kidneys and trachea were collected for histopathological evaluation. In IBV infected group, respiratory distress i.e., tracheal rales, nasal discharge, coughing, sneezing, gasping and reduced feed intake were observed during early stages, later up to 10 days post infection watery diarrhea with ruffled feathers were observed. In mix infected group clinical signs manifested rapidly and were persistent with
Summary
59
high severity. Gross lesions in mixed infection were more profound, including; severely congested and hyperemic lungs, tracheitis with catarrhal exudates in lumen; pale, swollen and enlarged kidneys with urates deposition in tubules. Six birds died in mix infected group, revealed caseous exudate in trachea extended up to lower bronchi while, in IBV infected group lesions were mild and confined to trachea, lungs and kidneys. Mortality was high in mix infected group (30%) followed by IBV infected group in which two birds died. While, in H9N2 infected and control group none of the bird died. Histopathological lesions in mix infected group were aggravated markedly tracheal epithelium degeneration and sloughing; congestion, interstitial nephritis, leukocytes infiltration, tubular degeneration and necrosis were observed while, in lungs pneumonia of peribronchiolar area and interstitium with lymphocyte and macrophages infiltration, additionally degeneration and vacuolization of hepatocytes with focal necrotic areas in liver were also noted. In IBV and H9N2 infected group microscopic lesions were of mild degree. GMTs against both IBV and H9N2 in mix infected group were significantly different at P>0.05 than individually infected birds. Among the groups, statistically significant increase in FCR of birds in mix infected group was observed. On the basis of these findings it might be conclude that mix infection of IBV and H9N2 causes severe disease as compared to single infection. Availability: Items available for loan: UVAS Library [Call number: 2296-T] (1).
9.
Effect of Fish Oil on Response of Lymphoid Organs of Broiler Experimentally Infected With Newcastle Disease Virus
by Muhammad Zahid (2013-VA-441) | Dr. Muhammad Yasin Tipu | Dr. Muhammad Yasin Tipu | Prof. Dr. Asim Aslam | Prof. Dr. Khushi Muhammad.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Theses submitted with cd. Availability: Items available for loan: UVAS Library [Call number: 2353-T] (1).
10.
Etiopathology Of Tuberculosis Complex In Antelopes And Its Cytokine Profile
by Maryam Saddiqa (2009-VA-366) | Dr. Raheela Akhtar | Dr. Muhammad Yasin Tipu | Dr. Ali Ahmed Sheikh.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2016Dissertation note: Bovine tuberculosis is a contagious disease of domestic and wild animals with serious zoonotic effects in humans. The economic importance and public health concern of bovine tuberculosis enlist it in the world top priority disease to be eliminated by WHO (World Health Organization). Tuberculosis in antelopes is caused by Mycobacterium bovis, Mycobacterium tuberculosis & Mycobacterium avium. This disease in antelopes causes tremendous economic losses. Unfortunately in Pakistan no such study has been conducted on wildlife tuberculosis except one (Zeeshan 2007) his work was on the identification of one specie i.e. M.bovis in deer while this study was on the identification of three different types of species (Mycobacterium bovis, Mycobacterium tuberculosis and Mycobacterium avium). A total of 50 blood samples from tuberculosis suspected antelopes were collected. These samples were subjected to multiplex polymerase chain reaction (multiplex PCR) and cytokine ELISA to determine the etiopathology of Mycobacterium tuberculosis complex.
The results indicated that 10% and 4% of antelopes were positive for M.bovis, M.tuberculosis infection with multiplex PCR and cytokine ELISA respectively. From these results it is evident that polymerase chain reaction (PCR) is more sensitive than cytokine ELISA for the diagnosis Mycobacterium Tuberculosis complex (MTC) and it shows much higher percentage of positive cases.
This study provided valuable information about the presence of M.bovis, M.tuberculosis and M.avium in different types of antelopes (Urial, Mouflon sheep, Black buck, Goral and Hog deer). The cytokine profile could be used as a diagnostic marker in future. Availability: Items available for loan: UVAS Library [Call number: 2549-T] (1).
11.
A Comparative Study Of Non-Antibiotic Feed Additives On Experimental Colonization Of Salmonella Enterica Serovar Enteridis And Intestinal Pathomorphology In Broiler Chickens
by Adeem Rehman Raffie (2010-VA-233) | Prof. Dr. Asim Aslam | Dr. Muhammad Yasin Tipu | Dr. Imran Altaf.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: The utility of antimicrobial agents as a preventive measure has been questioned, given extensive documentation of the evolution of antimicrobial resistance among pathogenic bacteria. Non-antibiotic feed additives (probiotics, prebiotics, essential oils and organic acids) are being considered to fill this gap and already a few farmers in the country are using them with good results. The present study enable us to understand and compare the beneficial effects of non-antibiotic feed additives on salmonella enterica colonization and changes in intestinal morphology.
This study is designed to evaluate the effect of non-antibiotic feed additives on salmonella enterica serovar enteritidis colonization in intestine of broilers chickens and compare the intestinal morphology between normal healthy, non-antibiotic feed additives supplemented and salmonella challenged broiler chickens.
A total of 125 commercial day-old broiler chicks were procured from the local market. The chicks were divided into six groups A (Basal diet, negative control group), B (Challenge + Basal diet, positive control group), C (probiotic + Challenge + Basal diet), D (prebiotic + Challenge + Basal diet), E (essential oils + Challenge + Basal diet) and F (organic acids + Challenge + Basal diet) with 20 chicks in each group and given separate treatments.
Two separate experiments were carried out for salmonella recovery from cecal tonsils and intestinal pathomorphic evaluation. Villus length, villus width, villus surface area and crypt depth were measured by micrometery. The collected data from both experiments was analyzed using the statistical technique of comparing more than two groups i.e. Analysis of variance (ANOVA) through SPSS 16.0.
Summary
45
There was an overall increase in all the parameters of intestinal morphometric analysis for all the treatment groups except for the control negative group which showed lowest values. Maximum villus height of 1794.2±63.96 μm in duodenum was achieved by group E, which was given essential oils. Whereas maximum villus surface area index of 1662.6±389.16 mm2 was recorded in group D, which was treated with prebiotics. Maximum villus height of 940.35±23.96 μm and surface area index of 568.92±36.27 mm2 in ileum mucosa was recorded in group D, treated with prebiotic. . Recoverable salmonella was most reduced by probiotics and organic acids.
Final results show that there is an overall increase in histological parameters of the mucosa of duodenum and ileum in the groups fed non-antibiotics feed additives as compared with control groups. Prebiotics showed the maximum positive effects in histological parameters whereas probiotics showed maximum positive effect for decreased recoverable salmonella count. Hence this study suggests that a combination of non-antibiotic feed additives will be beneficial for the intestinal health of broiler chickens but there is a need for more research on combinations of non-antibiotic feed additives. Availability: Items available for loan: UVAS Library [Call number: 2844-T] (1).
12.
Identification And Molecular Characterization Of Mycobacterium Bovis And Mycobacterium Paratuberculosis In Wild Cats.
by Zianab Tariq (2010-VA-234) | Dr. Muhammad Yasin Tipu | Prof. Dr. Zafar Iqbal Chaudhary | Prof. Dr. Aftab Ahmed Anjum.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Cases of wildlife diseased with Mycobacterium species are existing in Pakistan and result in high morbidity and mortality. Vaccine is the only preventive measure, but in wildlife the vaccine administration is a strenuous job. In Pakistan vaccination practice is not up to the mark and vaccination schedules are not being followed. Mycobacterial diseases have gain popularity due to their zoonotic effect. Scat samples from Lahore Safari Zoo and Lahore Zoo were collected and properly labelled. Conventional PCR along with Touchdown PCR was done using universal primer sets of M. bovis and M. paratuberculosis. The amplicons were run on agarose gel and the bands were observed under Gel Doc system.
The objective of the study was to detect the currently prevailing Mycobacterium bovis and mycobacterium avium subspecies paratuberculosis in wild cats in Pakistan.
However the results obtained from different kinds of PCR were negative, showing that the wild cat population of Lahore Zoo Safari as well as Lahore Zoo were free from Mycobacterium bovis and mycobacterium avium subspecies paratuberculosis. Availability: Items available for loan: UVAS Library [Call number: 2845-T] (1).
13.
Detection Of Genetic Variants In Interferon Gamma Gene And Its Association With Resistance Against Mycobacterium Bovis In Buffalo
by Awais Nawaz (2010-VA-219) | Prof. Dr. Asim Aslam | Dr. Muhammad Yasin Tipu | Dr. Jawad Nazir.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Bovine Tuberculosis (bovine TB) is a chronic disease of animals and has been known for the significant zoonotic impact. Immune mechanisms necessary for protection against Bovine TB are poorly understood. Interferon-γ cytokine has been reported critically and it is important to study its role in immunity against Bovine TB.
Blood samples were collected from 100 Animals from Peri-urban areas of Lahore, Gujranwala and Okara, Pakistan. Genomic DNA was extracted from the samples. Specific primers were designed to amplify specific portion of IFN-γ gene. Amplified products were sequenced and analyzed by bioinformatics tools. Interferon-γ assay was performed from blood collected in heparin coated vacutainers for the quantification of interferon-γ cytokine in different groups of animals. Blood samples from mycobacterium infected symptomatic and symptomatic animals were processed in Haematology analyzer for complete blood count.
Genetic sequencing of bovine Interferon gamma gene (IFN- γ) help in finding out the Genetic Variations to characterize its role in resistance against Mycobacterium bovis infection. This study help in finding out the confirmed markers for natural resistance against bovine TB that can be used in future selection and breeding programmes. The comparison of hematological values and Interferon gamma level of different groups of animals help us for the detailed diagnosis and prognosis of the disease.
The collected data from hematological analysis of Mycobacterium infected symptomatic animals (Group A), Mycobacterium infected asymptomatic animals (Group B) and non-infected animals/control Group (Group C) was analyzed using the statistical technique of comparing more than two groups i.e. Analysis of variance (ANOVA), One way ANOVA through SPSS 16.0.
CHAPTER 6
SUMMARY
Summary
71
Mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration were found non-significant (p>0.05). White blood cells, Lymphocytes, Platelets, Mean platelet volume and Mean corpuscular volume were found significant (p<0.05). Granulocytes, Red blood cells and Red cell distribution width values were found highly significant.
Interferon gamma assay provided confirmation about the presence of disease in the animals by indicating interferon gamma level to insight the undergoing pathogenesis which was helpful in the detailed diagnosis of the disease. Later on it helped us in the confirmation of false positive results by Tuberculin test.
Final results revealed four intronic variations in different groups of animals. Three of them were found in Group A and B and one was found in Group C (non-infected animals) by Primer 1 (P1). Intronic variations don’t have significant effect but they may have an impact on the regulation of the gene.
We found Transversions (T > A), (A > T), (T > G) were found in mycobacterium infected symptomatic group of animals (Table: 4.7). Transversion (C > G) at and deletion (G >_) was found in this group and exclusive presence of these SNP’s in this group can be considered significant and responsible for the infection. Transversion (A > C) and addition (_ > G) were found in mycobacterium infected asymptomatic group of animals. These two SNP’s are significant as they have been found only in this group. We can infer that the presence of these two SNP's is responsible for the infection along with making them asymptomatic towards the disease.
It was noted that Transition (G > A), (T > C) has been found common in mycobacterium infected symptomatic and asymptomatic group of animals. This common mutation at same position in both groups is quite significant and could be attributed to the occurrence of disease.
Summary Availability: Items available for loan: UVAS Library [Call number: 2881-T] (1).
